[TET proteins and epigenetic modifications in cancers]. Also known as: MDS; KIAA1546. Symbol: Tet2: Name: tet methylcytosine dioxygenase 2: RGD ID: 1311625: Description: Predicted to have ferrous iron binding activity; methylcytosine dioxygenase activity; and zinc Boster Bio Anti-TET2 Antibody catalog # A00191. TET Enzymes and 5-Hydroxymethylcytosine in Neural Progenitor Cell Biology and Neurodevelopment. Gagnon J, Daou S, Zamorano N, Iannantuono NV, Hammond-Martel I, Mashtalir N, Bonneil E, Wurtele H, Thibault P, Affar el B. Epigenetics. 2013 Sep;123(9):3672-7 TET2 is the most expressed TET gene in the hematopoietic tissue, especially in hematopoietic stem cells. Top Disease Cases with TET2 Mutation Nat Protoc. The double epigenetic modifications on both DNA and histones by TET2 and OGT coordinate together for the regulation of gene transcription. Mol Cell. Careers. Tet methylcytosine dioxygenase 2 (TET2) is a human gene. 2007;2:1445–1457. Analysis of Tet2 knockout mice, which are viable and fertile, demonstrated that Tet2 functions as a tumor suppressor whose haploinsufficiency initiates myeloid and lymphoid transformations. TET proteins, such as TET2, play key roles in the regulation of DNA-methylation status by oxidizing 5-methylcytosine (5mC) to generate 5-hydroxymethylcytosine (5hmC), which can both serve as a stable epigenetic mark and participate in active demethylation (Guallar et al., 2018). However, the molecular mechanism by which TET family enzymes regulate gene transcription remains elusive. Int J Mol Sci. The TET2 gene is the product of a … 2021 Mar 10;12(1):1556. doi: 10.1038/s41467-021-21661-y. Unable to load your collection due to an error, Unable to load your delegates due to an error. Fenwarth L, Caulier A, Lachaier E, Goursaud L, Marceau-Renaut A, Fournier E, Lebon D, Boyer T, Berthon C, Marolleau JP, Preudhomme C, Duployez N. Hemasphere. -, Ko M, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi. eCollection 2020. Nature. -, Robinson KA, Ball LE, Buse MG. Shin EM, Huynh VT, Neja SA, Liu CY, Raju A, Tan K, Tan NS, Gunaratne J, Bi X, Iyer LM, Aravind L, Tergaonkar V. Sci Adv. -. The TET family of proteins play a role in DNA modification, through the oxidation of methyl-cytosine, and in normal and malignant development. Supplied as 100ul in Liquid form antibody. Figure 2. Pasca S, Jurj A, Zdrenghea M, Tomuleasa C. Cancers (Basel). In this issue of Blood, Stremenova Spegarova et al report 3 patients with biallelic loss-of-function (LOF) TET2 mutations. An important paralog of this gene is TET1. Research Indicators 2013 Feb;14(2):64-5. doi: 10.1038/nrm3511. 2013 Mar 6;32(5):645-55. doi: 10.1038/emboj.2012.357. Polycythemia vera is frequently caused by mutations (changes) affecting the JAK2 gene, and less frequently by mutations affecting the TET2 gene. Prevention and treatment information (HHS). 2021 Mar 17;7(12):eabe2470. 2011;473:394–397. Am J Physiol Endocrinol Metab. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). 1 These patients suffered from infections, autoimmunity, and lymphoma, demonstrating 3 of the 5 potential phenotypes seen in inborn errors of immunity. Downregulation of TET2 reduces the amount of histone 2B Ser 112 GlcNAc marks in vivo, which are associated with gene transcription regulation. Consequently TET2 must be considered as a tumour suppressor gene. Optimal gene model selection by random forest. NCBI Description of TET2 The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. 2021 Feb 18;9:645335. doi: 10.3389/fcell.2021.645335. 2015;10(8):677-91. doi: 10.1080/15592294.2015.1060387. 2015 Dec;124(4):429-42. doi: 10.1007/s00412-015-0513-1. Epub 2016 Nov 15. These … The TET2 gene provides instructions for making a protein whose function is unknown. Epub 2015 Apr 18. 2015 Jan;263(1):6-21. doi: 10.1111/imr.12239. Clipboard, Search History, and several other advanced features are temporarily unavailable. 2013 Feb 21;49(4):645-56. doi: 10.1016/j.molcel.2012.12.019. This antibody reacts with Human, Mouse, Rat. National Library of Medicine Used to study myelodysplastic syndrome. -, Nature. 2014 Jun;22(3):836-41. doi: 10.7534/j.issn.1009-2137.2014.03.049. 2009;324:930–935. TET2 enhanced histone glycosylation, Figure 3. HIV-1 Vpr degrades TET2 by CRL4 E3 ligase to sustain IL-6 expression to enhance viral replication and disease progression. A critical perspective of the diverse roles of O-GlcNAc transferase in chromatin. The encoded protein is involved in myelopoiesis, and defects in this gene have been … FOIA TET2 is a tumor supressor and mutations of the gene are seen in myeloid malignancies and other hematological disorders. -, Shechter D, Dormann HL, Allis CD, Hake SB. 2021 Mar 27;22(7):3463. doi: 10.3390/ijms22073463. Matching is performed using the Specificity-Defining Region (SDR) [?] -, Blood. Garcia-Outeiral V, de la Parte C, Fidalgo M, Guallar D. Front Cell Dev Biol. 2011;473:389–393. TET2 is frequently mutated in myeloproliferative disorders (MPD). doi: 10.1126/sciadv.abe2470. GO annotations related to this gene include methylcytosine dioxygenase activity. Would you like email updates of new search results? These sequences represent the protein coding region of the TET2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. Science. 2021 Jan 18;8:630754. doi: 10.3389/fcell.2020.630754. 2021 Mar 10. doi: 10.1007/s12010-021-03537-5. TET2 (tet methylcytosine dioxygenase 2) is a protein-coding gene. Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Extraction, purification and analysis of histones. The anti- TET2 antibody was generated using TET2 as the antigen and it reacts with Human, Mouse, and Rat. Genetically engineered T cells that induced remission in a patient with chronic lymphocytic leukaemia were found to have disruption of the TET2 gene, which caused T … The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Ten-Eleven Translocation-2 (TET2) inactivation through loss-of-function mutation, deletion and IDH1/2 (Isocitrate Dehydrogenase 1 and 2) gene mutation is a common event in myeloid and lymphoid malignancies. -, Xu Y, et al. Gene TET2 TET2 Mutation is present in 3.76% of AACR GENIE cases, with lung adenocarcinoma, colon adenocarcinoma, cutaneous melanoma, acute myeloid leukemia, and myelodysplastic syndromes having the greatest prevalence [ 4 ]. Epub 2013 Jan 24. 8600 Rockville Pike The Complexity of TET2 Functions in Pluripotency and Development. Careers. Hereditary Predisposition to Acute Myeloid Leukemia in Older Adults. Epub 2014 Sep 3. Using protein affinity purification, here we search for functional partners of TET proteins, and find that TET2 and TET3 associate with O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT), an enzyme that by itself catalyses the addition of O-GlcNAc onto serine and threonine residues (O-GlcNAcylation) in vivo. Description The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. Nat Rev Mol Cell Biol. ▼ Cloning and Expression Please enable it to take advantage of the complete set of features! TET2. In murine models, the consequence of Tet2 inactivation is a depletion of 5hmC in bone marrow cells, an increase of the pool of HSCs, and an orientation of maturation towards monocyte/macrophage differentiation (Quivoron et … The TET2 level is regulated by interaction with IDAX, originating from TET2 gene fission during evolution, and by the microRNA miR-22. Please enable it to take advantage of the complete set of features! 8600 Rockville Pike The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. This antibody reacts with Human. 2015 Dec 16;69:1371-83. doi: 10.5604/17322693.1186346. 2017 Jan;105(1):17-22. doi: 10.1007/s12185-016-2122-z. Conversion of 5mC into 5hmC, 5fC and 5caC probably … This review summarizes the recently identified TET2 physiological and pathological functions and discusses how this knowledge influences our therapeutic approaches in hematological malignancies and possibly other tumor types. eCollection 2021 Apr. TETology: Epigenetic Mastermind in Action. Diseases associated with TET2 include refractory anemia, and blastic plasmacytoid dendritic cell. TET2 is the most expressed TET gene in the hematopoietic tissue, especially in hematopoietic stem cells. Would you like email updates of new search results? -, Pastor WA, et al. 2013 Jun;23(3):218-20 The gene view histogram is a graphical view of mutations across TET2. The TET2 gene comprises 11 exons spread over 150 kb and is widely expressed. Two variants encoding different isoforms have been found for this gene. Privacy, Help 2011 Oct 6;118(14 ):3932-41 Clipboard, Search History, and several other advanced features are temporarily unavailable. TET2 mediates DNA demethylation of the IL-6 promoter in osteosarcoma (OS) primary tumor cells. gene page. 2009;459:455–459. Gene type: protein coding. Location & Maps more. The following TET2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). TET2 regulates H2B S112 GlcNAc…, Figure 3. R01 CA130899/CA/NCI NIH HHS/United States, R01 CA132755/CA/NCI NIH HHS/United States, Tahiliani M, et al. Epub 2013 Jan 25. Sequence Map Chr3:133463677-133545136 bp, - strand From NCBI annotation of GRCm38. Front Cell Dev Biol. Tested in ELISA, IF, IHC, WB applications. However, the molecular mechanism by which TET family enzymes regulate gene transcription remains elusive. 2014 Sep;105(9):1093-9. doi: 10.1111/cas.12484. doi: 10.1097/HS9.0000000000000552. Epub 2015 Jun 22. TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS. Ten eleven translocation (TET) enzymes, including TET1, TET2 and TET3, convert 5-methylcytosine to 5-hydroxymethylcytosine and regulate gene transcription. Go to Variation Viewer for TET2 variants. Nature. The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. Accessibility Dysregulation of TET2 in hematologic malignancies. Vella P, Scelfo A, Jammula S, Chiacchiera F, Williams K, Cuomo A, Roberto A, Christensen J, Bonaldi T, Helin K, Pasini D. Mol Cell. Unable to load your collection due to an error, Unable to load your delegates due to an error. In addition to their hydroxylase activity, TET proteins recruit the O-linked β-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) enzyme to chromatin, which promotes post-transcriptional modifications of histones and facilitates gene expression. Tet2 Name. TET2 gene mutations similar to those observed in myeloid and lymphoid malignancies also accumulate with age in otherwise healthy subjects with clonal hematopoiesis. IUBMB Life. Ko M, An J, Pastor WA, Koralov SB, Rajewsky K, Rao A. Immunol Rev. Mendes K, Schmidhofer S, Minderjahn J, Glatz D, Kiesewetter C, Raithel J, Wimmer J, Gebhard C, Rehli M. Nat Commun. Ten eleven translocation (TET) enzymes, including TET1, TET2 and TET3, convert 5-methylcytosine to 5-hydroxymethylcytosine and regulate gene transcription. Based on the function of similar proteins, researchers believe the TET2 protein is involved in regulating the process of transcription, which is the first step in protein production. Seethy A, Pethusamy K, Chattopadhyay I, Sah R, Chopra A, Dhar R, Karmakar S. Appl Biochem Biotechnol. The TET2 interactors and their links to hematological malignancies. The epigenetic pioneer EGR2 initiates DNA demethylation in differentiating monocytes at both stable and transient binding sites. TETology: Epigenetic Mastermind in Action. Go to complete Gene record for TET2. Genomic Landscape and Risk Stratification in Chronic Myelomonocytic Leukemia. Nature. TET2 directly interacts with OGT, which is important for the chromatin association of OGT in vivo. Tested in IHC, WB applications. Cancer Sci. Alliance. Normal Function. Human ortholog(s) of this gene implicated in acute myeloid leukemia; chronic myelomonocytic leukemia; myelodysplastic syndrome; myeloproliferative neoplasm; and primary immunodeficiency disease. 2013 Feb 21;49(4):618-9. doi: 10.1016/j.molcel.2013.02.006. Nature. 2011;42:451–464. These mutations are displayed at the amino acid level across the full length of the gene by default. Alliance ; Gene:101887161 GREB1: An evolutionarily conserved protein with a glycosyltransferase domain links ERα glycosylation and stability to cancer. Seethy A, Pethusamy K, Chattopadhyay I, Sah R, Chopra A, Dhar R, Karmakar S. Appl Biochem Biotechnol. In addition to their hydroxylase activity, TET proteins recruit the O-linked β-D-N-acetylglucosamine (O-GlcNAc) transferase (OGT) enzyme to chromatin, which promotes post-transcriptional modifications of histones and facilitates gene expression. Zhongguo Shi Yan Xue Ye Xue Za Zhi. Supplied as 0.1 mg in Liquid form antibody. Online ahead of print. Online ahead of print. 2011;20:259–267. See all available tests in GTR for this gene. 2013 Aug 8;500(7461):222-6 Undetectable histone O-GlcNAcylation in mammalian cells. This site needs JavaScript to work properly. Show/Hide Columns or disappear Cell Line Depmap Id; Primary Disease; Disease Subtype Lineage Lineage Subtype; Chr; Start Position; End Position Variant … Melanoma Res. Gene ID: 54790, updated on 4-Oct-2020. JAK2 is known to provide the body with instructions to produce blood cells.When there is a mutation in this gene, the gene is constantly turned on, and the body therefore produces too many red blood cells. The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. TET2 is one of the three proteins of the TET (Ten-Eleven Translocation) family, which are evolutionarily conserved dioxygenases that catalyze the conversion of 5-methyl-cytosine (5-mC) to 5-hydroxymethyl-cytosine (5-hmC) and promote DNA demethylation. Accessibility TET proteins, such as TET2 (612839), regulate DNA-methylation status by oxidizing 5-methylcytosine (5mC) to generate 5-hydroxymethylcytosine (5hmC), which can both serve as a stable epigenetic mark and participate in active demethylation. Epub 2013 Jan 17. 2021 Mar 23;5(4):e552. 2010;468:839–843. 8 TSS. Different from the TET1 and TET3 genes, the human TET2 gene lacks the CpG DNA binding CXXC domain. TET proteins and 5-methylcytosine oxidation in hematological cancers. Reduction of O-GlcNAc protein modification does not prevent insulin resistance in 3T3-L1 adipocytes. Stem Cells Dev. Orthologous to human TET2 (tet methylcytosine dioxygenase 2). Although this specific interaction does not regulate the enzymatic activity of TET2, it facilitates OGT-dependent histone O-GlcNAcylation. TET2 (Tet Methylcytosine Dioxygenase 2, TET2) Short Description: The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. 2015 Jun;67(6):438-45. doi: 10.1002/iub.1389. TET2 as an epigenetic master regulator for normal and malignant hematopoiesis. -, J Clin Invest. TET2 regulates H2B S112 GlcNAc and gene transcription, National Library of Medicine Int J Hematol. Restrict the view to a region of the gene by dragging across the histogram to highlight the region of interest, or by using the sliders in the filters panel to the left. This site needs JavaScript to work properly. Using protein affinity purification, h … Deplus R, Delatte B, Schwinn MK, Defrance M, Méndez J, Murphy N, Dawson MA, Volkmar M, Putmans P, Calonne E, Shih AH, Levine RL, Bernard O, Mercher T, Solary E, Urh M, Daniels DL, Fuks F. EMBO J. Bethesda, MD 20894, Copyright TET2 has pleiotropic roles during hematopoiesis, including stem-cell self-renewal, lineage commitment and terminal differentiation of monocytes. Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. FOIA Postepy Hig Med Dosw (Online). -, Nature. Although this protein is found throughout the body, it may play a particularly important role in the production of … Print 2021 Mar. Boster Bio Anti-TET2 Antibody catalog # A00191-2. 2011;473:398–402. Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells. Nature. TET dioxygenases require 2-oxoglutarate, oxygen and Fe(II) for their activity, which is enhanced in the presence of ascorbic acid. Genome-wide regulation of 5hmC, 5mC, and gene expression by Tet1 hydroxylase in mouse embryonic stem cells. Collapse Section. Tet2 Gene Detail Summary Symbol. eCollection 2021. -, Wu H, et al. Tet proteins connect the O-linked N-acetylglucosamine transferase Ogt to chromatin in embryonic stem cells. -, Fujiki R, et al. 2021 Mar 10. doi: 10.1007/s12010-021-03537-5. 2014. -, Yang Z, et al. tet methylcytosine dioxygenase 2 ... MGI:2443298 NCBI Gene: 214133. Transcription Start Sites. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Curr Hematol Malig Rep. 2021 Mar 3. doi: 10.1007/s11899-021-00613-9. Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells. MicroRNA hsa-miR-138 inhibits adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells through adenovirus EID-1. Prevention and treatment information (HHS). Tet2 NCBI Gene records for discontinued versions of this gene: E130014J05Rik . shRNA constructs with 100% match to this gene. tet methylcytosine dioxygenase 2. Mol Cell. 2021 Mar 24;13(7):1499. doi: 10.3390/cancers13071499. See this image and copyright information in PMC. Taken together, these results reveal a TET2-dependent O-GlcNAcylation of chromatin. COSMIC gene TET2_ENST00000380013 (COSG77298) Genomic coordinates 4:105146786..105279803 (positive strand) Synonyms TET2, FLJ20032, KIAA1546, CCDS47120.1, Q6N021, ENSG00000168769.13, NM_001127208.2, NP_001120680 COSMIC-3D Couldn't retrieve COSMIC-3D data at this time Number of samples 75679 unique samples GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Chromosoma. 2007;292:E884–890. Online ahead of print. Moreover, OGT associates with TET2 at transcription start sites. Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. -, Ficz G, et al. It resides at chromosome 4q24, in a region showing recurrent microdeletions and copy-neutral loss of heterozygosity (CN-LOH) in patients with diverse myeloid malignancies. Privacy, Help TET2 was directly responsible for 5 hydroxymethylcytosine levels on osteogenic and adipogenic lineage-associated genes. Genome Resources. High-confidence TET2-associated genes exhibiting expression changes in PCa were identified as previously described [], and were assessed by random forest modeling in the training cohort to determine which of these genes would provide the best discriminatory power for prognostication of biochemical recurrence (BCR). Bethesda, MD 20894, Copyright O-GlcNAcylation and 5-methylcytosine oxidation: an unexpected association between OGT and TETs. 2012 Aug 30;488(7413):652-5 -.